Semiquantitative immunoblots of membrane protein-epidermal growth factor |
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Authors: | Richard Wing-Chuen Wong Dr Siu-Yuen Chan |
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Institution: | (1) Department of Paediatrics, the University of Hong Kong, Queen Mary Hospital, Hong Kong |
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Abstract: | Membrane proteins or cytokines are sometimes difficult to isolate and purify. Our group recently concentrated on epidermal
growth factor (EGF) protein expression studies. Mature EGF was initially identified from mouse submaxillary gland extract
as a stimulator of eyelid opening and incisor eruption when injected into newborn mice and rats. The EGF precursor is a transmembrane
protein with eight additional EGF-like repeats. Our previous study has shown that the EGF precursor without these eight EGF-like
repeats (hEGF) was biologically active. Here, we introduce a modified method for rapid detection of hEGF. The membranous protein
was directly extracted from various organs of transgenic mice (including the submandibular gland, kidney, liver, heart, and
testis) with two different buffers and easily detected by semiquantitative immunoblotting. |
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Keywords: | EGF protein extraction method immunoblot |
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