| 梨多倍体化对离体叶片不定梢再生能力的影响 |
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| 引用本文： | 孙清荣,孙洪雁,辛 力,李慧峰,刘庆忠.梨多倍体化对离体叶片不定梢再生能力的影响[J].植物遗传资源学报,2012,13(1):134-137. |
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| 作者姓名： | 孙清荣 孙洪雁 辛 力 李慧峰 刘庆忠 |
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| 作者单位： | 山东省果树研究所,泰安,271000 |
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| 基金项目： | 山东省自然科学基金(Y2008D50);山东省科技发展计划项目(2008GG2HZ09018) |
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| 摘 要： | 以源于二倍体梨品种Fertility(Pyrus communis L.)通过秋水仙碱离体诱变体细胞染色体加倍获得的不同同源多倍体无性系为试材,以离体叶片为外植体,观察研究了不同倍性无性系叶片的不定梢再生能力。结果表明,多倍体的不定梢再生率显著低于二倍体的再生率。不同多倍体无性系的不定梢再生能力也存在显著差异。三倍体无性系3x-3和四倍体无性系4x-4不能诱导产生不定梢。表明器官发生能力下降或植物细胞全能性的丧失与细胞染色体多倍体化有关。
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| 关 键 词： | 梨 多倍体 叶片 不定梢再生 细胞全能性 |
| 收稿时间： | 1/25/2011 4:48:45 PM |
| 修稿时间： | 5/11/2011 2:27:32 PM |
Effect of Polyploidization of Pear(Pyrus communis L.) on Shoot Regeneration Ability from in vitro Leaf Explants |
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| SUN Qing-rong
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SUN Hong-yan
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XIN Li
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LI Hui-feng
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LIU Qing-zhong.Effect of Polyploidization of Pear(Pyrus communis L.) on Shoot Regeneration Ability from in vitro Leaf Explants[J].Journal of Plant Genetic Resources,2012,13(1):134-137. |
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| Authors: | SUN Qing-rong SUN Hong-yan XIN Li LI Hui-feng LIU Qing-zhong |
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| Institution: | Shandong Institute of Pomology |
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| Abstract: | Shoot regeneration ability from in vitro leaf explants of different ploidy clones which were derived from the same diploid cultivar of pear(Pyrus communis L.)by colchicine treatment was examined.The regeneration rates of Neopolyploids were significantly lower than that of diploid control.Regeneration rates among different neopolyploid clones were significantly different.Triploid ’3x-3’ and tetraploid ’4x-4’ failed to regenerate.These results revealed that the decrease of regeneration ability or loss of totipotency was related to the polyploidization. |
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| Keywords: | Pyrus communis neopolyploid in vitro leaves shoot regeneration totipotency |
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