高粱材料耐盐性综合评价方法的初步建立与验证

用0、0.2%、0.4%、0.6%、0.8%5个浓度的NaCl溶液对10份高粱材料进行处理,测定了发芽盐害率、芽高盐害率、侧根数盐害率、根长盐害率、1/2叶片萎蔫持续时间和整个叶片萎蔫持续时间6项指标。以发芽期和苗期的各项指标为基础,根据每个指标盐害率制定各指标的得分方法,最后通过各个材料的得分判断其耐盐性,建立了一套高粱材料的耐盐评价标准。以此标准对10份高粱材料的耐盐性进行评价,排列出这10份高粱材料的耐盐次序,将高粱材料划分为4个耐盐等级。从这10份高粱材料中随机挑选5份耐盐性不同的材料通过盆栽试验对其耐盐性进行鉴定,以检验该评价方法的可靠性,结果表明:盆栽试验的鉴定结果与按照耐盐评分标准得出的结果一致,由此确定此评价方法运用于筛选高粱材料的耐盐性是准确、可靠的。同时在这10份高粱材料中筛选出了耐盐性较强的高粱材料河农1号和能饲1号。

Establishment and Verification of Comprehensive Evaluation Method for Salt Tolerance of Sorghum Materials

10 sorghum strains germinated under 0.0 %, 0.2%, 0.4%, 0.6%, 0.8% NaCl solution stress, the salt injury was evaluated with the injury of salt to the rate of germination, root length, the number of lateral roots, the duration of 1 / 2 leaf wilting and of the entire leaf wilting. Based on the changes of the above six indicators, the system of each index score for each indicator were developed. The salt tolerance of each sorghum strain was evaluated according to the total scores based on each indicator. A set salt-tolerant evaluation criteria was developed to evaluate salt tolerance. According to the degree of salt tolerance, these strains were divided into four levels. Five materials with different salt-tolerance from the ten sorghum strains were chosen to identify the salt tolerance with planting in saline soils contain 0.5% and 0.7% salt respectively in pots to verify the criteria. The results showed that the evaluation criteria was consistent with the test in pot, which confirmed the evaluation method used in the screening tolerant sorghum are reliable. Two high salt-tolerance sorghum strains, HN-1 and NS-1 were screened with this method.