| 60Co辐照对水稻基因组DNA诱变的分子生物学效应 |
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| 引用本文： | 向太和,杨剑波,杨前进,朱启升,李莉,倪大虎,汪秀峰,黄大年. 60Co辐照对水稻基因组DNA诱变的分子生物学效应[J]. 生物化学与生物物理进展, 2002, 29(5): 754-759 |
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| 作者姓名： | 向太和 杨剑波 杨前进 朱启升 李莉 倪大虎 汪秀峰 黄大年 |
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| 作者单位： | 浙江大学生命科学院，杭州310012;安徽省农业科学院水稻研究所，农业部水稻遗传育种重点开放实验室，合肥 230031;安徽省农业科学院水稻研究所，农业部水稻遗传育种重点开放实验室，合肥 230031;安徽省农业科学院绿色食品工程研究所，合肥 230031;安徽省农业科学院绿色食品工程研究所，合肥 230031;安徽省农业科学院水稻研究所，农业部水稻遗传育种重点开放实验室，合肥 230031;安徽省农业科学院水稻研究所，农业部水稻遗传育种重点开放实验室，合肥 230031;安徽省农业科学院水稻研究所，农业部水稻遗传育种重点开放实验室，合肥 230031;中国水稻研究所，杭州 310006;浙江大学生命科学院，杭州310012 |
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| 基金项目： | 国家自然科学基金(19890300)和安徽省自然科学基金资助项目（00041110）. |
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| 摘 要： | 以水稻品种农林8号及其60Co γ射线辐照突变体农林8号m为研究材料，选用360个10碱基寡核苷酸随机引物，利用随机扩增多态性DNA标记技术筛选出1个引物OPG18在农林8号和农林8号m之间表现出共显性的多态性.通过对该共显性标记的克隆和序列分析表明，突变体农林8号m与农林8号相比有29 bp DNA片段的缺失.研究结果为60Co γ射线辐照导致植物基因组DNA缺失提供了一个最直接明确的证据.
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| 关 键 词： | 辐照，水稻，基因组DNA，诱变，DNA缺失 |
| 收稿时间： | 2002-03-04 |
| 修稿时间： | 2002-03-04 |
Molecular Biological Effect of 60Co Gamma-ray Irradiation on Rice Genome DNA |
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| XIANG Tai-He,YANG Jian-Bo,YANG Qian-Jin,ZHU Qi-Sheng,LI Li,NI Da-Hu,WANG Xiu-Feng and HUANG Da-Nian. Molecular Biological Effect of 60Co Gamma-ray Irradiation on Rice Genome DNA[J]. Progress In Biochemistry and Biophysics, 2002, 29(5): 754-759 |
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| Authors: | XIANG Tai-He YANG Jian-Bo YANG Qian-Jin ZHU Qi-Sheng LI Li NI Da-Hu WANG Xiu-Feng HUANG Da-Nian |
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| Affiliation: | Life College of Zhejiang University, Hangzhou 310012, China;Rice Research Institute of Anhui Academy of Agricultural Sciences, Key Laboratory of Rice Genetics and Breeding of The National Agriculture Ministry, Hefei 230031, China;Rice Research Institute of Anhui Academy of Agricultural Sciences, Key Laboratory of Rice Genetics and Breeding of The National Agriculture Ministry, Hefei 230031, China;Natural Nourishing Food Engineering Institute of Anhui Academy of Agricultural Sciences, Hefei 230031, China;Natural Nourishing Food Engineering Institute of Anhui Academy of Agricultural Sciences, Hefei 230031, China;Rice Research Institute of Anhui Academy of Agricultural Sciences, Key Laboratory of Rice Genetics and Breeding of The National Agriculture Ministry, Hefei 230031, China;Rice Research Institute of Anhui Academy of Agricultural Sciences, Key Laboratory of Rice Genetics and Breeding of The National Agriculture Ministry, Hefei 230031, China;Rice Research Institute of Anhui Academy of Agricultural Sciences, Key Laboratory of Rice Genetics and Breeding of The National Agriculture Ministry, Hefei 230031, China;China National Rice Research Institute, Hangzhou 310006, China;Life College of Zhejiang University, Hangzhou 310012, China |
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| Abstract: | A total of 360 arbitrary 10-mer oligonucleotide primers were screened using RAPD (random amplified polymorphic DNA) technique on the genome DNA of Norin 8 and its 60Co gamma-ray irradiation mutant Norin 8m. One primer OPG18 produced co-dominant polymorphic markers OPG18/972 and OPG18/943 from Norin 8 and Norin 8m. Based on the clones and sequences of the OPG18/972 and OPG18/943, it shows that a 29 bp DNA fragment in the genome DNA of Norin 8m is deleted. The results directly confirmed the fact that 60Co gamma-ray irradiation can induced a bigger DNA fragment deletion of plant genome DNA. |
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| Keywords: | 60Co gamma-ray irradiation rice (oryza sative) genome DNA mutagenesis DNA fragment deletion |
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