Effect of hyperosmolarity on recombinant protein productivity in baculovirus expression system |
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Authors: | Olejnik A Grajek W Marecik R |
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Affiliation: | Department of Biotechnology and Food Microbiology, Agricultural University in Poznan, Wojska Polskiego 48 Street, Poznan, Poland. aolejnik@owl.au.poznan.pl |
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Abstract: | A lot of strategies were applied to improve recombinant protein productivity in the baculovirus expression system. In this study we propose for foreign protein production fed-batch cultivation method at hyperosmotic environment induced by increased NaCl content. Obtained results suggested relatively high tolerance and adaptation abilities of Tn-5 insect cells exposed to hyperosmotic stress. The cells under hyperosmotic conditions increased the specific rate of glucose consumption and lactate production. The release of additional energy and precursors as a result of increased metabolism by osmotically stressed culture was involved in recombinant protein synthesis. Recombinant nucleoprotein productivity in nutritional feeding cultures exposed to hyperosmolarity was about 72% higher than that obtained in batch culture at physiological osmolarity, but 31% was a result of feeding and the rest 41% was a result of hyperosmolarity and increasing Na(+) concentration. |
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