中华鳖4个Sox基因保守区的序列分析

采用PCR技术，扩增和克隆了中华鳖Sox基因（TSSox）。经DNA序列分析显示，Sox基因在系统进化上十分保守，其中TSSox4与鸟类LF4基因编码的氨基酸序列完全相同、与人类SOX4和Sox4编码的序列仅一个氨基酸的差异；TSSox5与鸟类的LF5基因的编码也仅一个氨基酸发生了改变；TSSox2与海龟的TSox2相似性最高。4条TSSox序列中，TSSox与人SRY基因序列相似性最高，达75％；序列上的相似性可能暗示了它们在功能上的保守性。

THE CONSERVATIVE REGION SEQUENCE ANALYSIS OF FOURSox GENES IN THE TRIONYX SINENSIS

The Sox genes of Trionyx sinensis were amplified and cloned using PCR. The four different fragments of Sox gene HMG-box DNA were selected and sequenced. The results indicated that the Sox gene is conservative in phylogeny, in which the amino acid sequence encode by TSSox4 (The Sox of T. Sinensis ) is identical to those encode by LF4 (bird) or differ from SOX4(human) and Sox4(mouse) only on one amino acid; The amino acid sequences encode by TSSox5 have an amino acid difference from those encode by LF5(bird); TSSox1 has about 75% identical to SRY HMG-box at the nucleotide level, and is most resemblance among four TSSox. Phylogenetic analysis of available 32 Sox sequences including SRY/Sry sequence suggests that there wolud be a high degree of divergence between any possible immediate common ancestor of the T. Sinensis Sox sequence and the SRY(Sry) sequences.