Response of the plant root to aluminum stress: Analysis of the inhibition of the root elongation and changes in membrane function |
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Authors: | Hideaki Matsumoto Yasushi Senoo Minobu Kasai Masayoshi Maeshima |
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Institution: | (1) Research Institute for Bioresources, Okayama University, 710 Okayama, Japan;(2) Department of Botany, Faculty of Science, Hirosaki University, 036 Aomori, Japan;(3) Department of Agricultural Chemistry, Nagoya University, 464-01 Nagoya, Japan |
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Abstract: | Pea root elongation was strongly inhibited in the presence of a low concentration of Al (5 μM). In Al-treated root, the epidermis
was markedly injured and characterized by an irregular layer of cells of the root surface. Approximately 30% of total absorbed
Al accumulated in the root tip and Al therein was found to cause the inhibition of whole root elongation. Increasing concentrations
of Ca2+ effectively ameliorated the inhibition of root elongation by Al and 1 mM of CaCl2 completely repressed the inhibition of root elongation by 50 μM Al. The ameliorating effect of Ca2+ was due to the reduction of Al uptake.
H+-ATPase and H+-PPase activity as well as ATP and PPidependent H+ transport activity of vacuolar membrane vesicles prepared from barley roots increased to a similar extent by the treatment
with 50 μM AlCl3. The rate of increase of the amount of H+-ATPase and H+-PPase was proportional to that of protein content measured by immunoblot analysis with antibodies against the catalytic subunit
of the vacuolar H+-ATPase and H+-PPase of mung bean. The increase of both activities was discussed in relation to the physiological tolerance mechanism of
barley root against Al stress. |
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Keywords: | Al stress Barley H+-ATPase and H+-PPase Localization of Al Pea Root elongation |
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