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The human erythrocyte membrane skeleton may be an ionic gel. III. Micropipette aspiration of unswollen erythrocytes
Authors:B T Stokke  A Mikkelsen  A Elgsaeter
Institution:1. Internal Medicine Department, State Medical University, Zaporozhye, Ukraine;2. Centre for Chronic Disease Prevention and Management, College of Health and Biomedicine, Victoria University, St Albans, Australia;3. Molecular and Cellular Therapeutics Department, Royal College of Surgeons in Ireland, Dublin, Ireland;4. Department of Histology and Embryology, School of Medicine, University of Ljubljana, Ljubljana, Slovenia;5. International Clinical Research Center, St. Anne''s University Hospital, Masaryk University, Brno, Czech Republic;1. University of Siena, Italy;2. University of Catania, Italy;3. U Sod di Psichiatria, AOU Careggi Firenze, Italy;4. UOSD S.P.D.C. P.O. Giulianova Asl Teramo, Italy;5. University of Cagliari, Italy;6. University of Milano Bicocca, Italy;7. ASL Salerno, EBRIS foundation, Italy;8. University Magna Graecia, Catanzaro, Italy;9. Neuropsychiatric clinic villa von Siebenthal-Rome, Italy;10. Department of Mental Health, ASL Lecce, Italy;11. Department of Mental Health, Ulss2 Marca Trevigiana, Italy;12. ASL Roma 6, Rome, Italy;13. Area Vasta2, Ancona-A.S.U.R.Marche, Italy;14. Otsuka Pharmaceuticals, Italy;15. University of Palermo, Italy;p. University of Torino, Italy;q. DSMD - Neuroscienze Asst Fatebenefratelli- Sacco, Milano, Italy;r. Lundbeck, Italy;s. ASST G.O.M. Niguard, Milano, Italy;t. University of Padova Medical Center, Italy;u. University of Perugia, Italy;v. University of Brescia, Italy
Abstract:We have carried out a theoretical analysis of micropipette aspiration of unswollen erythrocytes using the protein-gel-lipid-bilayer membrane model and taking into account that the modulus of area compression of the membrane skeleton may depend on the environmental conditions. Our analysis shows that the aspiration pressure needed to obtain a certain membrane projection length is strongly dependent on the ratio between the membrane skeleton modulus of area compression and the elastic shear modulus. Our analysis therefore predicts that micropipette aspiration of unswollen erythrocytes may be a sensitive method for detection of changes in this ratio. The analysis thus also shows that micropipette aspiration of unswollen erythrocytes can not be used to determine the membrane shear modulus unless something is known about the membrane skeleton modulus of area compression.
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