Cytoskeleton changes following differentiation of N1E-115 neuroblastoma cell line |
| |
Authors: | J-E Oh K Karlmark Raja J-H Shin A Pollak M Hengstschläger G Lubec |
| |
Institution: | (1) Department of Pediatrics, Medical University of Vienna, Vienna, Austria;(2) Prenatal Diagnosis and Therapy, Department of Obstetrics and Gynecology, Medical University of Vienna, Vienna, Austria |
| |
Abstract: | Summary. No systematic approach to detect expression of differentiation-related elements was published so far. The undifferentiated
N1E-115 neuroblastoma cell line was switched into a neuronal phenotype by DMSO treatment and used for proteomic experiments.
We used two-dimensional gel electrophoresis followed by unambiguous mass spectrometrical identification of proteins to generate
a map of cytoskeleton proteins (CPs), i.e., to search for differentiation-related structures. Alpha-actin, actin-like protein
6A, gamma-tubulin complex component 2, tubulin alpha 3/alpha 7, CLIP associating protein 2, B4 integrin interactor homolog
were detectable in the undifferentiated cell line exclusively and neuron-specific CPs drebrin and presynaptic density protein
95, actin-related protein 2/3, alpha and beta-centractin, PDZ-domain actin binding protein, actinin alpha 1, profilin II,
ezrin, coactosin-like protein, transgelin 2, myosin light polypeptide 6, tubulin alpha 2, 6 and 7, beta tubulin (94% similar
with tubulin beta-2), tubulin beta 3, tubulin tyrosine ligase-like protein 1, lamin B1 and keratin 20 were observed in the
differentiated cell line only. We herein identified differentiation-related expressional patterns thus providing new evidence
for the role of CPs in the process of neuronal differentiation. |
| |
Keywords: | : Cytoskeleton proteins – Actin/actin binding protein – Tubulin – Proteomics – Differentiation – N1E-115 cell line |
本文献已被 PubMed SpringerLink 等数据库收录! |
|