Lifeact: a versatile marker to visualize F-actin |
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| Authors: | Riedl Julia Crevenna Alvaro H Kessenbrock Kai Yu Jerry Haochen Neukirchen Dorothee Bista Michal Bradke Frank Jenne Dieter Holak Tad A Werb Zena Sixt Michael Wedlich-Soldner Roland |
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| Affiliation: | Max Planck Institute of Biochemistry, Independent Junior Research Group Cellular Dynamics and Cell Patterning, Am Klopferspitz 18, 82152 Martinsried, Germany. |
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| Abstract: | Live imaging of the actin cytoskeleton is crucial for the study of many fundamental biological processes, but current approaches to visualize actin have several limitations. Here we describe Lifeact, a 17-amino-acid peptide, which stained filamentous actin (F-actin) structures in eukaryotic cells and tissues. Lifeact did not interfere with actin dynamics in vitro and in vivo and in its chemically modified peptide form allowed visualization of actin dynamics in nontransfectable cells. |
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