Involvement of transposable elements in the formation of hybrid phages between bacteriophage P1 and the R plasmid NR1 |
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| Authors: | Shigeru Iida Jürg Meyer Katsutoshi Mise Werner Arber |
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| Institution: | Department of Microbiology, Biozentrum, University of Basel, Klingelbergstrasse 70, CH-4056 Basel, Switzerland;Department of Microbiology, The Institute of Public Health, 4-6-1, Shirokanedai Minato-Ku, Tokyo 108, Japan |
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| Abstract: | Homologous recombination between IS1 elements present on both replicons, P1 and NR1, resulted in P1-NR1 cointegrates and P1-RTF and P1-r-det phages. Cointegration between P1 and NR1-B, and NR1 derivative with multiple DNA rearrangements including insertion of the transposable element γδ, was also mediated by reciprocal recombination in IS1 sequences. However, all 4 hybrids studied carried deletions promoted by γδ residing on NR1-B. Further IS1-mediated deletions on the hybrid genomes resulted in plaque-forming P1Cm phages. |
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| Keywords: | DNA rearrangement Cointegration Deletion R plasmid Bacteriophage P1 Transposable element IS1 |
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