Microbes involved in dissimilatory nitrate reduction in the human large intestine |
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Authors: | Parham Gibson |
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Affiliation: | Microbiology Department, Institute of Food Research, Earley Gate, Reading RG6 6BZ UK |
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Abstract: | Nitrate-limited batch cultures, incorporating 20 different fermentation substrates and inoculated with human faeces, mainly selected for the growth of enterobacteria. The microbial diversity involved was determined by a combination of phenotypic and genotypic procedures. Continuous culture with lactate as the sole electron donor selected for similar micro-organisms, but when antibiotics were incorporated to inhibit Escherichia coli and lactate was replaced with choline, there was a wider microbial diversity recovered. Clostridium ramosum and Bacteroides vulgatus were then isolated as well as enterobacteriaceae. |
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Keywords: | Colonic bacterium Nitrate reduction Enterobacteria |
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