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DIFFERENT RESULTS BY DIFFERENT COMMERCIAL TAQ DNA POLYMERASE IN RAPD
Authors:HE Li  YUAN Shuiquan  MO Banghui  YAO Guanggui  LIN Honghui  CHEN Fang  GUO Xiaocai
Abstract:RAPD (Random Amplified Polymorphic DNA) technique has been widely used in animal, plant, human and microorganism research since it was first established by Williams in 1990[1-3]. But, because of low annealed temperature and short 10-nt primers, the resolution and repetition is low in RAPD. The stability of RAPD is influenced by many factors such as the concentration of template, primers, dNTP, Mg++,and Taq DNA polymerase[4-6]. The influence on amplified products of different commercial Taq DNA polymerase in RAPD was studied in this paper.
Keywords:commercial Taq DNA polymerase  RAPD  fragments amplified  influence
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