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| 基于粪便DNA的雪豹种群调查和遗传多样性 | |
| 引用本文： | 张于光,Jan E.JANECKA,李迪强,朵海瑞,R.JACKSON,W.J.MURPHY. 基于粪便DNA的雪豹种群调查和遗传多样性[J]. 动物学报, 2008, 54(5) |
| 作者姓名： | 张于光 Jan E.JANECKA 李迪强 朵海瑞 R.JACKSON W.J.MURPHY |
| 作者单位： | 1. 中国林业科学研究院森林生态环境与保护研究所,国家林业局森林生态环境重点实验室,北京 100091 2. Department of Veterinary Integrative Biosciences, College of Veterinary Medicine and Biomedical Sciences, Texas A&M University, TX 77843-4458, USA 3. Snow Leopard Conservancy, Sonoma, California 95476, USA |
| 基金项目： | 国家科技支撑计划，科技部科技基础条件平台建设计划 |
| 摘 要： | 雪豹 (Panthera uncia) 是仅分布于亚洲高海拔山区的珍稀濒危猫科动物.本研究在印度西南部(Ladakh)、中国青海和蒙古国的南部(南Gobi)3个独立的雪豹分布区共采集109份粪便样品.应用线粒体DNA(mtDNA) cyt b基因特异性引物对109份粪便样品进行鉴定,发现有31份粪便来自雪豹,其中印度Ladakh、我国青海和蒙古国南Gobi的雪豹样品分别为17份、3份和11份.利用重新筛选设计的7对家猫(Felis catus)微卫星引物,对雪豹粪便样品进行了基因分型分析,结果发现在Ladakh和南Gobi检测到的雪豹粪便样品分别来自4只和5只不同的雪豹个体,而青海的样品则来自同一只雪豹;遗传多样性统计分析表明,蒙古国南Gobi的雪豹微卫星遗传多样性水平低于印度的Ladakh.研究结果表明了粪便DNA在雪豹种群监测和遗传多样性研究中的可行性. |
| 关 键 词： | 雪豹 粪便DNA 遗传多样性 种群 |
Population survey and genetic diversity of snow leopards Panthera uncia as revealed by fecal DNA |
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| ZHANG Yu-Guang,Jan E.JANECKA,LI Di-Qiang,DUO Hai-Rui,R.JACKSON,W.J.MURPHY. Population survey and genetic diversity of snow leopards Panthera uncia as revealed by fecal DNA[J]. Acta Zoologica Sinica, 2008, 54(5) | |
| Authors: | ZHANG Yu-Guang Jan E.JANECKA LI Di-Qiang DUO Hai-Rui R.JACKSON W.J.MURPHY |
| Affiliation: | ZHANG Yu-Guang1,Jan E.JANECKA2,LI Di-Qiang1,DUO Hai-Rui1,R.JACKSON3,W.J.MURPHY21.Institute of Forest Ecology,Environment , Protection,, the Key Laboratory of Forest Ecology , Environment of State Forestry Administration,Chinese Academy of Forestry,Beijing 100091,China2.Department of Veterinary Integrative Biosciences,College of Veterinary Medicine , Biomedical Sciences,Texas A&M University,College Station,TX 77843-4458,USA3.Snow Leopard Conservancy,Sonoma,California 95476,USA |
| Abstract: | Snow leopards Panthera uncia occur in rugged,high altitude regions of Central Asia,where they are endangered as a result of human induced factors including low prey densities and poaching.Information on the status of this felid is limited in many regions.We examined the feasibility of using noninvasive genetic methods to monitor snow leopard populations and to study their genetic diversity.We observed snow leopard scats in three separate geographic regions during brief surveys and collected 109 scats from s... |
| Keywords: | Snow leopard Panthera uncia Fecal DNA Genetic diversity Population |
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