| 利用CLSM检测GFP基因在家蚕的瞬时表达 |
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| 引用本文： | 陈智毅,李清兵,陈列辉,廖森泰,李宝瑜,廖琼香.利用CLSM检测GFP基因在家蚕的瞬时表达[J].遗传,2000,22(5):303-304. |
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| 作者姓名： | 陈智毅 李清兵 陈列辉 廖森泰 李宝瑜 廖琼香 |
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| 作者单位： | 广东省农业科学院蚕业研究所,广东五山 510640
Sericultural Institute of Guangdong,Guangzhou 510640,China |
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| 基金项目： | 广东省自然科学基金!资助项目(编号960573)。本文承蒙方定坚、邢达、余迪求教授审阅，谨此致谢。 |
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| 摘 要： | 将绿色荧光蛋白(GFP)基因导入农蚕蛹(G0)的睾丸,利用激光共聚焦扫描显微镜(CLSM)系统检测,得到GFP基因在家蚕刚孵出未进食的幼虫(蚁蚕)(G1)瞬时表达的荧光图像。
Abstract:Plasmid DNA containing the GFP(Green Fluorescent Protein)reporter gene was injected into the testis of silkworm(Bombyx mori L.)pupa(G0),we observe the fluorescence imaging of expression of GFP gene in silkworm newly-hatched larvae(G1)examined by Confocal Laser Scanning Microscope.
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| 关 键 词： | 转基因 绿色荧光蛋白 共聚焦扫描显微镜 Key words Bombyx mori L. 家蚕 |
Expression of GFP Gene in Silkworm Examined by CLSM |
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| CHEN Zhi-yi,LI Qing-bing,CHEN Lie-hui,LIAO Seng-tai,LIBao-yu,LIAO Qiong-xiang.Expression of GFP Gene in Silkworm Examined by CLSM[J].Hereditas,2000,22(5):303-304. |
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| Authors: | CHEN Zhi-yi LI Qing-bing CHEN Lie-hui LIAO Seng-tai LIBao-yu LIAO Qiong-xiang |
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| Abstract: | Plasmid DNA containing the GFP(Green Fluorescent Protein)reporter gene was injected into the testis of silkworm(Bombyx mori L.)pupa(G0),we observe the fluorescence imaging of expression of GFP gene in silkworm newly hatched larvae(G1)examined by Confocal Laser Scanning Microscope. |
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| Keywords: | silkworm (Bombyx mori L ) gene transfer green fluorescent protein(GFP) confocal laser scanning microscope (CLSM) |
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