A restriction map of the bacteriophage T4 genome |
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Authors: | Patrick H O'Farrell Elizabeth Kutter and Mikiye Nakanishi |
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Institution: | (1) Department of Biochemistry, University of California, 94143 San Francisco, CA, USA;(2) The Evergreen State College, 98505 Olympia, Wash, USA |
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Abstract: | Summary We report a detailed restriction map of the bacteriophage T4 genome and the alignment of this map with the genetic map. The sites cut by the enzymes BglII, XhoI, KpnI, SalI, PstI, EcoRI and HindIII have been localized. Several novel approaches including two-dimensional (double restriction) electrophoretic separations were used. |
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