Preparation of poly( -lysine) adsorbents and application to selective removal of lipopolysaccharides |
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Authors: | Chuichi Hirayama Masayo Sakata Mariko Nakamura Hirotaka Ihara Masashi Kunitake Masami Todokoro |
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Affiliation: | a Department of Applied Chemistry & Biochemistry, Faculty of Engineering, Kumamoto University, 2-39-1 Kurokami, Kumamoto 860-8555, Japan;b Graduate school of Science & Technology, Kumamoto University, 2-39-1 Kurokami, Kumamoto 860-8555, Japan;c Chisso Co. Ltd., 1-1 Noguchi-Cho, Minamata-Shi, Kumamoto 867-8501, Japan |
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Abstract: | To remove endotoxins (lipopolysaccharides; LPS) from cell products used as drugs, water-insoluble poly( -lysine) (PL) particles were prepared by cross-linking with PL originating from Streptomyces albulus and chloromethyloxirane (CMO). The apparent pKa (pKa,app) and the anion-exchange capacity of the particles were easily adjusted by changing the PL ratio and the CMO ratio. The higher the pKa,app, the greater the LPS-adsorption capacity of the particles. On the other hand, when the PL ratio (in the particles) increased to 75 unit-mol% or higher, the adsorption of bovine serum albumin by the particles also increased, but decreased with increasing ionic strength of the buffer to μ=0.2 or higher. The adsorption of γ-globulin increased with decreasing PL ratio to 65 unit-mol% or lower. As a result, when the PL ratio was 70 unit-mol% and the pKa,app was 6.7, the PL/CMO particles selectively removed LPS from various protein solutions that were naturally contaminated with LPS, at pH 6.0 and μ=0.05. |
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Keywords: | Lipopolysaccharides Endotoxins Poly( http://www.sciencedirect.com/scidirimg/entities/25b.gif" alt=" var epsilon" title=" var epsilon" border=" 0" >-lysine) |
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