Simultaneous on-line extraction and analysis of sirolimus (rapamycin) and ciclosporin in blood by liquid chromatography–electrospray mass spectrometry |
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| Authors: | Gabriele I Kirchner Christian Vidal Wolfgang Jacobsen Anke Franzke Katrin Hallensleben Uwe Christians Karl-Friedrich Sewing |
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| Institution: | a Institut für Allgemeine Pharmakologie, Medizinische Hochschule Hannover, Carl-Neuberg-Str.1, D-30625 Hannover, Germany;b Institut für Hämatologie und Onkologie, Medizinische Hochschule Hannover, Carl-Neuberg-Str.1, D-30625 Hannover, Germany;c Department of Biopharmaceutical Sciences, School of Pharmacy, University of California, San Francisco, CA, USA |
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| Abstract: | We developed a sensitive and specific semi-automated liquid chromatography–electrospray mass spectrometric (HPLC–ESI-MS) assay for the simultaneous quantification of sirolimus and ciclosporin in blood. Following a simple protein precipitation step, the supernatants were injected into the HPLC system and extracted on-line. After column switching, the analytes were backflushed from the extraction column onto the analytical narrow-bore column and eluted into the ESI-MS system. The assay was linear from 0.4 to 100 μg/l sirolimus and from 2 to 1500 μg/l ciclosporin. The mean recoveries of sirolimus and ciclosporin were 98 and 96%, respectively. The mean interday precision/accuracy was 8.6%/−4.8% for sirolimus and 9.3%/−2.9% for ciclosporin. |
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| Keywords: | Sirolimus Rapamycin Ciclosporin |
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