Improvements in the phosphoramidite procedure for the synthesis of oligodeoxyribonucleotides. |
| |
Authors: | T Drper and E L Winnacker |
| |
Institution: | T Dörper and E L Winnacker |
| |
Abstract: | The paper describes an improved method for the synthesis of oligodeoxyribonucleotides using phosphoramidite chemistry. Our procedure relies on novel phosphoramidite intermediates, the deoxyribonucleoside-3'-morpholine-methoxyphosphins. These compounds are extremely stable and can be purified readily. Condensation reactions during solid-phase synthesis can thus be performed with high efficiency and result in a high yield synthesis of long chain oligodeoxyribonucleotides. |
| |
Keywords: | |
|