Nondenaturing protein electrotransfer of the esterase activity of lipolytic preparations |
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| Authors: | M C Brahimi-Horn M L Guglielmino A M Gaal L G Sparrow |
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| Institution: | Division of Wool Technology, Melbourne Laboratory, Parkville, Australia. |
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| Abstract: | Lipolytic enzymes subjected to nondenaturing polyacrylamide gel electrophoresis were electrophoretically transferred under nondenaturing conditions onto a solid-state matrix. Electrotransfer permitted the visualization of hydrolytic activity to the long-chain water insoluble substrate alpha-naphthyl palmitate. Four commercial preparations: a lipase from Candida cylindracea, an esterase from porcine liver, a lipase from Pseudomonas sp., and a cholesterol esterase from Pseudomonas fluorescens were examined. |
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