Biodegradation of mixture containing monohydroxybenzoate isomers by <Emphasis Type="Italic">Acinetobacter calcoaceticus</Emphasis> |
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Authors: | K Prathibha Suresh Sumathi |
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Institution: | (1) School of Biosciences and Bioengineering, Indian Institute of Technology-Bombay, Powai, Mumbai, 400 076, Maharashtra, India;(2) Centre for Environmental Science and Engineering, Indian Institute of Technology-Bombay, Powai, Mumbai, 400 076, Maharashtra, India |
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Abstract: | A bacterial strain capable of utilizing a mixture containing 2-hydroxybenzoic acid (2-HBA), 3-hydroxybenzoic acid (3-HBA)
and 4-hydroxybenzoic (4-HBA) acid was isolated through enrichment from a soil sample. Based on 16SrDNA sequencing, the microorganism
was identified as Acinetobacter calcoaceticus. The sequence of biodegradation of the three isomers when provided as a mixture (0.025%, w/v each) was elucidated. The dihydroxylated
metabolites formed from the degradation of 2-HBA, 3-HBA and 4-HBA were identified as catechol, gentisate and protocatechuate,
respectively, using the cell-free supernatant and cell-free crude extracts. Monooxygenases and dioxygenases that were induced
in the cells of Acinetobacter calcoaceticus in response to growth on mixture containing 2-HBA, 3-HBA and 4-HBA could be detected in cell-free extracts. These data revealed
the pathways operating in Acinetobacter calcoaceticus for the sequential metabolism of monohydroxybenzoate isomers when presented as a mixture. |
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Keywords: | Acinetobacter calcoaceticus 2-hydroxybenzoic acid 3-hydroxybenzoic acid 4-hydroxybenzoic acid Oxygenases |
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