愈伤组织继代接种量、继代周期和年龄对软紫草原生质体脱壁的影响

本文的实验结果表明：软紫草愈伤组织脱壁所需的适宜酶浓度为：０．１％果胶酶十０．２５％纤维素酶；酶解处理的适宜时间与愈伤组织年龄有关：愈伤组织的适宜年龄随其继代周期、愈伤组织继代接种量而有变化。当接种量，１克／瓶，转代后７天进行原生质体分离：接种量３克／瓶，酶解材料则以培养５天愈伤组织为宜。继代周期１３天和１５天的比１５天和１５天的，适宜脱壁的愈伤组织当代培养天数要提前１天。

EFFECTS OF SUBCULTURE INOCULUM QUANTITY,SUBCULTURE TIME AND AGE OF CALLUS ON PROTOPLAST ISOLATION OF ARNEBIA EUCHROMA

The result showed:0.1% Pectinase+0.25% Onozuka R-10 was of benefit to isolate calli of A.euchroma;the approriate time of enzyme treatment was related to callus age.Callus age would vary if either subculture time or callus inoculum quantity was changed.The experiment material of isolating protoplast was 7-day callus when inoculum quantity was 1 g/flask,but 5-day callus when inoculum quantity was 3 g/flask. As the suitable material of enzyme treatment,callus of 15-day and 15-day subculture period might be cultured a fittle longer than that of 13-day and 15-day subculture period.