盐酸阿比朵尔抗柯萨奇病毒B3的实验研究

为观察盐酸阿比朵尔对柯萨奇病毒的抑制作用,本实验以利巴韦林为阳性对照药物,采用细胞培养技术、细胞病变效应(CPE)抑制法、活细胞染色计数法(MTT)和培养上清中病毒滴度测定观察盐酸阿比朵尔抗柯萨奇病毒的作用。结果表明盐酸阿比朵尔的半数中毒浓度(TD50)为896.54μg/mL,药物抗病毒生物合成组、药物直接作用组、药物抗病毒吸附2h组、药物抗病毒有吸附8h组的病毒抑制率分别能达到74.48%、45.68%、28.90%和48.27%,在抗生物合成组,盐酸阿比朵尔能明显抑制柯萨奇病毒所致的CPE效应,降低培养上清中的病毒滴度,病毒抑制率随药物浓度增加而增高,存在量效关系。这提示盐酸阿比朵尔在细胞内对柯萨奇病毒有一定的抑制作用。

Inhibitory Effect of Arbidol Hydrochloride on Coxsackievirus B_3 Group in vitro

To study the antiviral activity of Arbidol hydrochloride on Coxsackievirus Group B_3 (CVB_3), the antiviral effect of Arbidol hydrochloride were evaluated with virus inhibitory rates by MTT assay and 50% tissue culture infection dose (TCID_(50)) of the supernatant. The results showed that the viral inhibitory rates were up to 75.48%,45.68%,28.90% and 48.27% respectively in interrupting viral biosynthesis group, directly killing virus group, interfering in viral absorption and penetration groups(2h and 8h). In interrupting viral biosynthesis group,Arbidol hydrochloride could significantly inhibit cytopathic effect (CPE) of CVB_3-infected HEp-2 cells, increase survival rates of CVB_3-infected cells in dose dependent manner and decrease viral titers of the supernatant. It indicates that Arbidol hydrochloride has antiviral effect against Coxsackievirus group B3 (CVB_3).