Influence of glycosylation inhibitors on dihydropyridine binding to cardiac cells |
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Authors: | Henning Ursula Wallukat Gerd Holtzhauer Martin |
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Institution: | (1) Institute of Biochemistry and Molecular Physiology, University of Potsdam, Germany;(2) Department of Molecular Cardiology, Max Delbrück Center for Molecular Medicine, R.-Rössle-Str 10, D-13122 Berlin, Germany;(3) c/o Max Delbrück-Centrum, Robert-Rössle-Str. 10, D-13122 Berlin, Germany |
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Abstract: | In primary cultures of neonatal rat heart cells we found a linear correlation between the number of L-type calcium channel-specific dihydropyridine (DHP) binding sites and spontaneous beating frequency (v).Formation of glycoproteins in tissue culture was suppressed by different inhibitors of N-glycosylation. This inhibition alters to a different extent the binding of the DHP ligand (+)-methyl-3H]PN 200-110 and v. The most severe but reversible effect occurs at 6 g/ml tunicamycin (Bmax 45% and v 6%, resp., of control), a slight increase in Bmax at 0.1–0.5 mM castanospermine and 0.05–2.5 mM deoxymannojirimycin. The other inhibitors gave no significant alterations of Bmax. |
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Keywords: | calcium channel glycosylation inhibitor cultured cardiac cells dihydropyridine receptors glycoproteins |
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