A convenient method for the ATPase assay. |
| |
| Authors: | D LeBel G G Poirier A R Beaudoin |
| |
| Institution: | 1. Département de biologie, Université de Sherbrooke, Sherbrooke, Québec, Canada J1K 2R1;2. Unité de Gastroentérologie, Université de Sherbrooke, Sherbrooke, Québec, Canada J1K 2R1 |
| |
| Abstract: | A new method for the determination of inorganic phosphorus released in ATPase assay has been evaluated. The method is based on the reduction of a phosphomolybdate complex by Elon in a copper acetate buffer. In contrast to current methods, there is no interference by ATP with color development. There is also less or no interference by other compounds usually present in ATPase assay media. The method is simple, sensitive, and reproducible. |
| |
| Keywords: | |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
