Isolation, purification and characterization of a DPP-III homologue from goat brain |
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Authors: | Dhanda Suman Singh Hari Singh Jasbir Singh Tej P |
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Affiliation: | Department of Biochemistry, Kurukshetra University, Kurukshetra 136119, Haryana, India. dhandasuman@rediffmail.com |
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Abstract: | A dipeptidyl peptidase (DPP) from goat brain has been purified. The purified enzyme showed a single band on sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE). It is a monomer with molecular weight of 69kDa with a pI of 4.5. The K(m) was estimated to be 39microM for Arg-Arg-4-methoxy-beta-naphthylamide (Arg-Arg-4mbetaNA). This enzyme is strongly inhibited by commonly used metallochelators and sulfhydryl reagents. Among various beta-naphthylamides examined, Arg-Arg-4mbetaNA was the most rapidly hydrolyzed substrate. Although, initially it was thought to be the DPP-III but on the basis of its molecular weight and inhibition studies, it was concluded that this enzyme is a functional homologue of DPP-III. |
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Keywords: | Dipeptidyl peptidase III homologue Metalloenzyme Goat brain Arg-Arg-4-methoxy-β -naphthylamide DPP |
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