Inward rectifier K+ channel Kir2.3 is localized at the postsynaptic membrane of excitatory synapses |
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Authors: | Inanobe Atsushi Fujita Akikazu Ito Minoru Tomoike Hitonobu Inageda Kiyoshi Kurachi Yoshihisa |
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Institution: | Department of Pharmacology II, Graduate School of Medicine, Osaka University, Suita, Osaka 565-0871, Japan. |
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Abstract: | Classical inwardly rectifyingK+ channels (Kir2.0) are responsible for maintaining theresting membrane potential near the K+ equilibriumpotential in various cells, including neurons. Although Kir2.3 is knownto be expressed abundantly in the forebrain, its precise localizationhas not been identified. Using an antibody specific to Kir2.3, weexamined the subcellular localization of Kir2.3 in mouse brain. Kir2.3immunoreactivity was detected in a granular pattern in restricted areasof the brain, including the olfactory bulb (OB). Immunoelectronmicroscopy of the OB revealed that Kir2.3 immunoreactivity wasspecifically clustered on the postsynaptic membrane of asymmetricsynapses between granule cells and mitral/tufted cells. Theimmunoprecipitants for Kir2.3 obtained from brain contained PSD-95 andchapsyn-110, PDZ domain-containing anchoring proteins. In vitro bindingassay further revealed that the COOH-terminal end of Kir2.3 isresponsible for the association with these anchoring proteins.Therefore, the Kir channel may be involved in formation of the restingmembrane potential of the spines and, thus, would affect the responseof N-methyl-D-aspartic acid receptor channels atthe excitatory postsynaptic membrane. |
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