Effect of selenium deficiency on the disposition of plasma glutathione |
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| Authors: | K E Hill R F Burk |
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| Affiliation: | 1. Michael Barber Centre for Mass Spectrometry, Manchester Institute of Biotechnology, University of Manchester, Princess Road, Manchester M1 7DN, UK;2. ChELSI Institute, Department of Chemical and Biological Engineering, University of Sheffield, Mappin Street, Sheffield S1 3JD, UK;3. Department of Molecular Biology and Biotechnology, University of Sheffield, Western Bank, Sheffield S10 2TN, UK;4. Manchester Pharmacy School, University of Manchester, Stopford Building, Oxford Road, Manchester M13 9PT, UK;1. Department of Biochemistry, Quaid-e-Azam University, Islamabad, Pakistan;2. National Center of Excellence in Physical Chemistry, University of Peshawar, Peshawar, Pakistan;3. Department of Basic Sciences, College of Veterinary and Animal Sciences Jhang (Jhang Campus), University of Veterinary and Animal Sciences, Lahore, Pakistan;4. Department of Chemistry, University of Lahore, Lahore, Pakistan;1. Abington Jefferson Health, Abington, USA;2. Icahn school of medicine at Mount Sinai Elmhurst Hospital New York, USA;3. Rawalpindi Institute of Cardiology, Rawalpindi, Pakistan;4. University of South Dakota, Sioux Falls, USA;5. St Mary Medical Center, Langhorne, PA, USA;6. Thomas Jefferson University, Philadelphia, USA;7. Detroit Medical Center, DMC Heart Hospital, Detroit, MI, USA |
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| Abstract: | Selenium deficiency causes increased hepatic synthesis and release of GSH into the blood. The purpose of this study was to examine the effect of selenium deficiency on the disposition of plasma glutathione. Plasma glutathione concentration was 40 +/- 3.4 nmol GSH equivalents/ml in selenium-deficient rats and 17 +/- 5.4 nmol GSH equivalents/ml in control rats. The half-life and systemic clearance of plasma glutathione were found to be the same in selenium-deficient and control rats (t1/2 = 3.4 +/- 0.7 min). Because selenium-deficient plasma glutathione concentration was twice that of control, the determination that selenium deficiency did not affect glutathione plasma systemic clearance indicated that the flux of glutathione through the plasma was doubled by selenium deficiency. It has been proposed that the kidney is responsible for the removal of a major fraction of plasma glutathione. In these studies, renal clearance accounted for 24% of plasma systemic glutathione clearance in controls and 44% in selenium-deficient rats. This indicates that a significant amount of glutathione is metabolized at extrarenal sites, especially in control animals. More than half of the increased plasma glutathione produced in selenium deficiency was removed by the kidney. Thus, selenium deficiency results in a doubling of cysteine transport in the form of glutathione from the liver to the periphery as well as a doubling of plasma glutathione concentration. |
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