Distribution patterns and coexistence of neurohormonal peptides (ANP,BNP, NPY,SP, CGRP,enkephalins) in chromaffin cells and nerve fibers of the anuran adrenal organ |
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Authors: | M. Reinecke C. Heym W. G. Forssmann |
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Affiliation: | (1) Institute of Anatomy, University of Zürich, Winterthurer Strasse 190, CH-8057 Zürich, Switzerland;(2) Institut für Anatomie I der Universität, Im Neuenheimer Feld 307, W-6900 Heidelberg, Germany;(3) Niedersächsisches Institut für Peptidforschung (IPF), Feodor-Lynen-Strasse 5, W-3000 Hannover 61, Germany |
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Abstract: | Summary Immunohistochemical techniques were used to study the adrenal organs of the anuran species Rana esculenta, Caldula pulchra and Bufo marinus with respect to the distribution and coexistence of atrial natriuretic peptide (ANP), brain natriuretic peptide (BNP), substance P (SP), calcitonin gene-related peptide (CGRP), neuropeptide Y (NPY), Leu-enkephalin (Leu-ENK). Met-enkephalin-Arg-Phe (MEAP) and dynorphin A 1–17 (DYN). Antisera against enzymes involved in catecholamine synthesis, i.e., dopamine- -hydroxylase (DBH) and tyrosine hydroxylase (TH), were used for the identification of chromaffin cells. ANP-immunoreactive (-IR) cells occurred in high densities (30%–70% of the total cell population) in all species investigated. In C. pulchra and B. marinus, BNP-IR cells constituted a population of non-DBH-IR and non-TH-IR cells that were different from the ANP-IR cells. A large proportion of the adrenal cells (10%–55%) were immunoreactive to Leu-ENK, and a minority (2%–5%) showed MEAP-immunoreactivity. DYN-immunoreactivity was not observed. The anurans studied exhibited small numbers of SP-IR, CGRP-IR and NPY-IR cells. Immunoreactivities for ANP+Leu-ENK and Leu-ENK+ MEAP were shown to coexist. In C. pulchra and B. marinus, immunoreactions for ANP+NPY, ANP+SP and SP+CGRP were also colocalized. Except for DYN, all neurohormonal peptides also occurred in intra-adrenal nerve fibers. SP-IR fibers also displayed CGRP-immunoreactivity and some Leu-ENK-IR fibers contained MEAP-immunoreactivity. In C. pulchra, NPY-IR fibers were found that also showed ANP-immunoreactivity.Some results of this investigation have been presented in abstract form (Reinecke et al. 1991). |
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Keywords: | Adrenal organ Atrial natriuretic peptides Neuropeptide Y Substance P Calcitonin gene-related peptide Enkephalins Catecholamine synthesizing enzymes Rand esculenta, Caldula pulchra, Bufo marinus (Anura) |
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