Biotin biosynthetic pathway in recombinant strains of Escherichia coli overexpressing bio genes: evidence for a limiting step upstream from KAPA |
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Authors: | Sophie Lévy-Schil Laurent Debussche Sylvie Rigault Fabienne Soubrier Fabrice Bacchetta Delphine Lagneaux Josiane Schleuniger Francis Blanche Joël Crouzet Jean-François Mayaux |
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Affiliation: | (1) Unité de Biologie Moléculaire, Institut de Biotechnologies, Rhône-Poulenc Rorer S.A., Centre de Recherche de Vitry-Alfortville, 13 Quai Jules Guesde, B.P. 14, 94403 Vitry sur Seine Cédex, France;(2) Department Analyses, Rhône-Poulenc Rorer S.A., Centre de Recherche de Vitry-Alfortville, 13 Quai Jules Guesde, B.P. 14, 94403 Vitry sur Seine Cédex, France;(3) Present address: 4 rue Hubert Vaillant, 60950 Ver sur Launette, France |
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Abstract: | The effect of the overexpression of the bioABFCD operon on the biotin biosynthetic pathway was investigated in an Escherichia coli K12 bioR mutant with a chromosomal deletion for the biotin operon. When transformed with a multicopy number plasmid containing bioABFCD, this strain synthetized 10,000 times more biotin than a wild-type E. coli strain. In order to further increase biotin production, the bioA and bioB operons were subcloned into plasmids with stronger promoters and in some cases optimal ribosome binding sites. The new constructions led to the accumulation of large amounts of soluble Bio proteins (although not BioC) but did not improve biotin production. In all the constructed strains, BioA, BioD, and BioB activities were greatly amplified but these activitie did not correlate with the level of protein syntthesis. These strains accumulated only low levels of vitamers, auggesting that the major limiting step for higher biotin production occurs upstream from the first intermediate of the Bio pathway we assayed (7,keto-8-aminopelargonic acid). As BioC overproduction was shown to impair cell growth, we could not determine if this early step of pathway was limiting.Correspondence to: S. Lévy-Schil |
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