Cloning and expression of the Mycobacterium fortuitum Superoxide dismutase gene |
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Authors: | M Carmen Menéndez Pilar Domenech Jesus Prieto María J García |
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Institution: | Institute for Experimental Pathology, University of Iceland, Keldur, 112 Reykjavík, Iceland |
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Abstract: | Abstract The beta-toxin gene from Clostridium perfringens type C was cloned and expressed as a glutathione S-transferase fusion protein in Escherichia coll . The DNA sequence was determined and compared to the type B sequence. Two nucleotide differences were found in the protein coding sequence, resulting in one amino acid difference between the two proteins. The purified beta-toxin fusion protein is not toxic in mice, but rabbit antiserum raised against it neutralises the toxic effect of C. perfringens type C culture filtrate in mice. |
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Keywords: | Clostridium perfringens Beta-toxin Fusion protein Neutralizing antiserum |
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