Screening for the presence of the insertion sequence IS1 in the genus Bacteroides |
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| Authors: | MJ Larkin GW Blakely DR Williams S Patrick |
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| Institution: | Sub-Department of Microbiology, The Queen's University of Belfast, David Keir Building, Belfast BT7, 1NN, U.K.;Department of Microbiology and Immunobiology, The Queen's University of Belfast, Grosvenor Road, Belfast, BT12 6BN, U.K. |
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| Abstract: | Abstract A specific DNA probe, containing a conserved region of the insertion sequence IS1, was hybridised to dot blots of total genomic DNA from 2 oral and 5 intestinal Bacteroides spp. Using Escherichia coli K12 as a positive control and Pseudomonas aeruginosa as a negative control, DNA homologous to the probe could not be detected in Bacteroides corporis, Bacteroides intermedius, Bacteroides ovatus, Bacteroides vulgatus, Bacteroides thetaiotaomicron or 2 strains of Bacteroides fragilis . The total DNA included plasmid DNA of 30.2, 42.7 and 42.7 MDa from B. fragilis, B. intermedius and B. corporis , respectively.
IS1 is commonly found in members of the Enterobacteriaceae, and it was concluded that the 2 groups of bacteria are not closely related. |
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| Keywords: | Bacteroides sp insertion sequence IS1 |
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