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Differential binding of chromosomal proteins HMG1 and HMG2 to superhelical DNA
Authors:M Bustin  N Soares
Institution:1. Department of Biochemistry, Vanderbilt University School of Medicine, Nashville, TN 37232, USA;2. Department of Biological Sciences, Vanderbilt University, Nashville, TN 37232, USA;1. Department of Biochemistry, Ludwig-Maximilians-University, 81377 Munich, Germany;2. Gene Center, Ludwig-Maximilians-University, 81377 Munich, Germany;3. Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, MA 02115, USA;4. Department of Biochemistry and Molecular Biophysics, Washington University School of Medicine, Saint Louis, MO, USA;5. Howard Hughes Medical Institute;1. Department of Biochemistry, Vanderbilt University School of Medicine, Nashville, TN 37232, USA;2. Department of Biological Sciences, Vanderbilt University, Nashville, TN 37232, USA
Abstract:The binding of chromosomal proteins HMG1 and HMG2 to various DNA structures was examined by a nitrocellulose filter binding assay using a 32P labelled supercoiled plasmid. Binding assays and competition experiments indicated that HMG2 has a higher affinity than HMG1 for supercoiled DNA. Studies at various ionic strengths and pH values reveal differences in the interaction of the two proteins with DNA. The results suggest that HMG1 and HMG2 are involved in distinguishable cellular functions.
Keywords:
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