Introduction of single-stranded ADH genes into Drosophila results in tissue-specific expression |
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Authors: | N L Shen W H Sofer |
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Institution: | Waksman Institute, Rutgers-State University of New Jersey, Piscataway 08855. |
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Abstract: | We injected single-stranded circular DNA containing a Drosophila Adh gene into ADH-negative embryos of Drosophila melanogaster and performed ADH histochemical staining on third instar larvae of the injected generation. Introduction of either the coding or non-coding strand resulted in correct tissue-specific expression of the Adh gene in larvae. Southern blotting revealed that the bulk of the injected DNA became double-stranded shortly after injection and was not integrated into the genome. |
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