A thermostable endo-1,4-/B-glucanase from Myceliophthora thermophila |
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| Authors: | Anatole A Klyosov Olga V Ermolova Vladimir M Chernoglazov |
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| Institution: | (1) The Carbohydrates Research Laboratory, A.N. Bach Institute of Biochemistry, USSR Academy of Sciences, 117071 Moscow, USSR;(2) Department of Chemistry, Moscow State University, 117234 Moscow, USSR |
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| Abstract: | Summary Endo-1,4-/B-glucanase from a thermophilic fungusMyceliophthora thermophila has been isolated and purified to homogeneity. The molecular weight of the enzyme was 52,000 with a pI of 4.7, pH-optimum 5.0–6.0, and specific activity 61 IU/mg (40°, pH 5.0); this activity is 2.4 times higher than that of the enzyme fromTrichoderma reesei. The new endoglucanase is very thermostable; its half-life at 65°C is 170 hours, which is 300 times higher than that of the enzyme fromT. reesei. |
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