| 白腐菌产漆酶的纯化及部分酶学性质 |
| |
| 引用本文： | 康从宝,刘巧,李清心,曲音波,高培基. 白腐菌产漆酶的纯化及部分酶学性质[J]. 中国生物化学与分子生物学报, 2002, 18(5): 638-642 |
| |
| 作者姓名： | 康从宝 刘巧 李清心 曲音波 高培基 |
| |
| 作者单位： | 1. 山东大学微生物技术国家重点实验室,济南,250100;大庆石油学院石油工程系,安达,151400
2. 山东大学微生物技术国家重点实验室,济南,250100 |
| |
| 基金项目： | 国家自然科学基金资助项目 (No .2 0 0 770 15 ) |
| |
| 摘 要： | 对白腐菌W 1产生的漆酶粗酶液通过超滤浓缩、分子筛和离子交换层析进行纯化 .用SDS PAGE证明该酶的分子量大约为 6 2 4kD .等电聚焦电泳显示该酶的等电点为 3 5 .酶反应的最适温度为 5 0℃ ,最适pH值为 4 5 .此酶氧化DMP的Km 值为 3 84× 10 -5mol L .金属离子对酶活的影响很大 ,其中K+ 、Mn2 + 、Ag+ 对酶活有促进作用 ,Fe2 + 、Fe3 + 、Hg2 + 、Co2 + 、Ba2 + 等对酶活有明显的抑制作用 .酶对部分染料也有一定的脱色效果
|
| 关 键 词： | 白腐菌 漆酶 纯化 酶学性质 |
| 收稿时间： | 2002-10-20 |
| 修稿时间： | 2001-12-05 |
Purification and Properties of Laccase Produced from a White Rot Fungus (W-1) |
| |
| KANG Cong-bao ),),LIU Qiao ),LI Qing-xin ),QU Yin-bo ),GAO Pei-ji ). Purification and Properties of Laccase Produced from a White Rot Fungus (W-1)[J]. Chinese Journal of Biochemistry and Molecular Biology, 2002, 18(5): 638-642 |
| |
| Authors: | KANG Cong-bao ) ) LIU Qiao ) LI Qing-xin ) QU Yin-bo ) GAO Pei-ji ) |
| |
| Affiliation: | ( 1)State Key Laboratory of Microbial Technology, Shandong University, Jinan 250100, China;= 2)Department of Petroleum Engineering, Daqing Petroleu |
| |
| Abstract: | The laccase was purified from a white rot fungus by ultrafiltration, Bio-gel P-100 and DEAE-Sephadex A-50 chromatography. The molecular weight of the purified laccase was determined as about 62^4 kD by SDS-PAGE. Its pI was determined to be 3.5 by IFE-PAGE. The optimum pH and temperature for laccase activity were 4.5 and 50℃, respectively. The laccase catalyzed the oxidation of 2,6-dimethoxyphenol with a Michaelis constant of 3.84×10 -5 mol/L. Various metal ions showed different effects on the activity. The activity was enhanced by K +, Mn 2+ and Ag +,and was strongly inhibited by Fe 2+, Fe 3+, Hg 2+, Co 2+ and Ba 2+. The laccase also had the ability to decolorize some dyes such as Remazol brilliant blue R and carmoisine. |
| |
| Keywords: | white rot fungus laccase purification enzymatic properties |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
| 点击此处可从《中国生物化学与分子生物学报》浏览原始摘要信息 |
|
点击此处可从《中国生物化学与分子生物学报》下载全文 |
|
