Multicolour whole-mount in situ hybridization to Drosophila embryos |
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Authors: | G Hauptmann Thomas Gerster |
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Institution: | Biozentrum der Universit?t Basel, Abteilung Zellbiologie, Klingelbergstrasse 70, CH-4056 Basel, Switzerland, CH
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Abstract: | We report an extended whole-mount in situ hybridization procedure for Drosophila embryos. By using probes labelled with digoxigenin, fluorescein and biotin, respectively, this protocol allows the detection
in three colours of RNAs derived from three different genes. Hybridized probes are detected by consecutive staining with appropriate
alkaline phosphatase conjugates using different chromogenic substrate combinations, and serial removal of the antibody conjugates
by low pH washes.
Received: 7 May 1996/Accepted: 7 July 1996 |
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Keywords: | Biotin-UTP Alkaline phosphatase substrates Fast Red Fast Blue B Magenta-phos/INT |
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