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Recombinant Sendai virus induces T cell immunity against respiratory syncytial virus that is protective in the absence of antibodies |
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| Authors: | Voges Brigitte Vallbracht Simone Zimmer Gert Bossow Sascha Neubert Wolfgang J Richter Kirsten Hobeika Elias Herrler Georg Ehl Stephan |
| Affiliation: | a Institut für Virologie, Stiftung Tierärztliche Hochschule Hannover, Bünteweg 17, 30559 Hannover, Germany b Zentrum für Kinderheilkunde und Jugendmedizin, Universitätsklinik Freiburg, Childrens Hospital, Mathildenstrasse 1, 79106 Freiburg, Germany c Max-Planck-Institut für Biochemie, Am Klopferspitz 18, 82152 Martinsried, Germany d Institut für med. Mikrobiologie und Hygiene, Universitätsklinik Freiburg, Hermann-Herder-Strasse 11, 79104 Freiburg, Germany e Max-Planck-Institut für Immunbiologie, Stübeweg 51, 71908 Freiburg, Germany |
| Abstract: | Respiratory syncytial virus (RSV) causes severe respiratory disease in infants and a vaccine is highly desirable. The fusion (F) protein of RSV is an important vaccine target, but the contribution of F-specific T cells to successful vaccination remains unclear. We studied the immune response to vaccination of mice with a recombinant Sendai virus expressing RSV F (rSeV F). rSeV F induced protective neutralizing antibody and RSV F-specific CTL responses. T cell immunity was stronger than that induced by recombinant vaccinia virus (rVV F), a well characterized reference vector. Vaccination of antibody-deficient mice showed that vaccine-induced RSV F-specific T cells were sufficient for protective immunity. rSeV F induced T cell immunity in the presence of neutralizing antibodies, which did not impair the vaccine response. Although the F protein only contains a subdominant CTL epitope, vaccination with rSeV F is sufficient to induce protective T cell immunity against RSV in mice. |
| Keywords: | Virus T cell Vaccine Lung Cellular immunity |
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