A self-assembled clavanin A-coated amniotic membrane scaffold for the prevention of biofilm formation by ocular surface fungal pathogens |
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| Authors: | Santi M. Mandal Jahangir Khan Denial Mahata Suman Saha Jayangshu Sengupta Osmar N. Silva |
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| Affiliation: | 1. Central Research Facility, Indian Institute of Technology Kharagpur, Kharagpur, Indiamandalsm@gmail.com;3. Central Research Facility, Indian Institute of Technology Kharagpur, Kharagpur, India;4. Rubber Technology Centre, Indian Institute of Technology Kharagpur, Kharagpur, India;5. Priyamvada Birla Aravind Eye Hospital, Kolkata, India;6. S-Inova Biotech, Pos-Gradua??o em Biotecnologia, Universidade Católica Dom Bosco, Campo Grande, Brazil |
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| Abstract: | Amniotic membrane (AM) is frequently used in ophthalmologic surgery for rapid ocular surface reconstruction. Sometimes it may create a major problem with associated infections after biofilm formation over the membrane. To overcome this problem, AM was coated with the antimicrobial peptide clavanin A. The antifungal activity of clavanin A in the native and self-assembled form was determined against the common ocular surface pathogens Candida albicans, Aspergillus fumigatus, Alternaria sp. and Fusarium sp. Biofilm formation over the coated surface was significantly reduced in comparison with the uncoated membrane. The coated membrane revealed effectiveness in terms of biocompatibility, cell attachment colonization when tested in non-cancerous 3T3 and human embryonic kidney (HEK)-293 cell lines. Clavanin A-coated AM also exhibited excellent physical, morphological and antifungal characteristics, indicating potential applicability for ocular surface infection control. |
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| Keywords: | Amniotic membrane antimicrobial peptides anti-biofilm clavanin A self-assembled |
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