Fast corroding,thin magnesium coating displays antibacterial effects and low cytotoxicity |
| |
Authors: | Sarah Zaatreh David Haffner Madlen Strauß Katharina Wegner Mareike Warkentin Claudia Lurtz |
| |
Affiliation: | 1. Biomechanics and Implant Technology Research Laboratory, Department of Orthopedics, University Medicine Rostock, Rostock, Germany;2. Inorganic Functional Materials, Institute of Materials Science, Faculty of Engineering, Kiel University, Kiel, Germany;3. Faculty of Mechanical Engineering and Marine Technology, Department of Material Science and Medical Engineering, University of Rostock, Rostock, Germany |
| |
Abstract: | Bacterial colonisation and biofilm formation are characteristics of implant-associated infections. In search of candidates for improved prosthetic materials, fast corroding Mg-based coatings on titanium surfaces were examined for their cytotoxic and antimicrobial properties. Human osteoblasts and Staphylococcus epidermidis were each cultured on cylindrical Ti samples coated with a thin layer of Mg/Mg45Zn5Ca, applied via magnetron sputtering. Uncoated titanium samples served as controls. S. epidermidis was quantified by counting colony forming units. The biofilm-bound fraction was isolated via ultrasonic treatment, and the planktonic fraction via centrifugation. Biofilm-bound S. epidermidis was significantly decreased by approximately four to five orders of magnitude in both Mg- and Mg45Zn5Ca-coated samples after seven days compared to the control. The osteoblast viability was within the tolerance threshold of 70% stated in DIN EN ISO 10993-5:2009-10 for Mg (~80%) but not for Mg45Zn5Ca (~25%). Accordingly, Mg-coated titanium was identified as a promising candidate for an implant material with antibacterial properties and low cytotoxicity levels. The approach of exploiting fast corrosion contrasts with existing methods, which have generally focused on reducing corrosion. |
| |
Keywords: | Implant-associated infections antibacterial coatings magnesium Staphylococcus epidermidis human osteoblasts |
|
|