Biofilm characteristics and evaluation of the sanitation procedures of thermophilic Aeribacillus pallidus E334 biofilms |
| |
| Authors: | Tugba Kilic Basar Karaca Beste Piril Ozel Birgul Ozcan Cumhur Cokmus |
| |
| Institution: | 1. Faculty of Science, Biology Department, Ankara University, Ankara, Turkey;2. Faculty of Sciences and Letters, Biology Department, Mustafa Kemal University, Hatay, Turkey;3. Faculty of Agriculture and Natural Sciences, Molecular Biology and Genetics Department, Konya Food and Agriculture University, Konya, Turkey |
| |
| Abstract: | The ability of Aeribacillus pallidus E334 to produce pellicle and form a biofilm was studied. Optimal biofilm formation occurred at 60 °C, pH 7.5 and 1.5% NaCl. Extra polymeric substances (EPS) were composed of proteins and eDNA (21.4 kb). E334 formed biofilm on many surfaces, but mostly preferred polypropylene and glass. Using CLSM analysis, the network-like structure of the EPS was observed. The A. pallidus biofilm had a novel eDNA content. DNaseI susceptibility (86.8% removal) of eDNA revealed its importance in mature biofilms, but the purified eDNA was resistant to DNaseI, probably due to its extended folding outside the matrix. Among 15 cleaning agents, biofilms could be removed with alkaline protease and sodium dodecyl sulphate (SDS). The removal of cells from polypropylene and biomass on glass was achieved with combined SDS/alkaline protease treatment. Strong A. pallidus biofilms could cause risks for industrial processes and abiotic surfaces must be taken into consideration in terms of sanitation procedures. |
| |
| Keywords: | Aeribacillus thermophilic bacilli biofilm formation biofilm removal EPS |
|
|
