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Molecular characteristics,biofilm-forming abilities,and quorum sensing molecules in Vibrio parahaemolyticus strains isolated from marine and clinical environments in Korea
Authors:Md. Furkanur Rahaman Mizan  Hyeon-Jo Bang  Mohammad Sadekuzzaman  Nari Lee  Tae-Jo Kim
Affiliation:1. Department of Food Science and Technology, Advanced Food Safety Research group, Brain Korea 21 Plus, Chung-Ang University, Anseong, Republic of Korea;2. Food Safety Research Group, Korea Food Research Institute, Seongnam-si, Republic of Korea;3. College of Education, Hospitality, Health and Human Sciences, Wisconsin’s Polytechnic University, Menomonie, WI, USA
Abstract:Vibrio parahaemolyticus is an inhabitant of marine and estuarine environments and causes seafood-borne gastroenteritis in humans. In this study, an UltraFast LabChip Real-Time PCR assay was evaluated for rapid detection and quantification of pathogenic V. parahaemolyticus isolates. Escherichia coli and Vibrio harveyi were used as negative controls. Twenty-six tdh-positive, biofilm-producing V. parahaemolyticus isolates were analyzed by repetitive extragenic palindromic-polymerase chain reaction (REP-PCR). REP-PCR analysis showed that the majority of the V. parahaemolyticus isolates originated from seafood and that clinical specimens formed two major clusters at 92.8% and 32% similarity levels. The presence and quantification of Autoinducer-2 was carried out using high-performance liquid chromatography with fluorescence detection (HPLC-FLD) after derivatization of Autoinducer-2 with 2, 3-diaminonaphthalene. The presence of tdh-positive V. parahaemolyticus in marine samples highlights the need for constant environmental monitoring to protect public health.
Keywords:Vibrio parahaemolyticus  REP-PCR  AI-2  HPLC-FLD  UltraFast LabChip Real-Time PCR
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