| FFA作用于脂肪细胞后细胞炎症因子的表达及变化 |
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| 引用本文： | 徐文静,张君,陆环,谢菁,李爱琴,郑丽英,李宏,谢建新. FFA作用于脂肪细胞后细胞炎症因子的表达及变化[J]. 现代生物医学进展, 2010, 10(1) |
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| 作者姓名： | 徐文静 张君 陆环 谢菁 李爱琴 郑丽英 李宏 谢建新 |
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| 作者单位： | 石河子大学医学院基础医学系病理生理学教研室/石河子大学《新疆地方与民族高发病》教育部重点实验室,新疆,石河子,832002 |
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| 基金项目： | 新疆生产建设兵团医药专项(2007GG25) |
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| 摘 要： | 目的:用FFA刺激脂肪细胞并在不同时间点收集细胞培养液,探讨FFA水平升高后对脂肪细胞的影响。方法:将3T3-L1细胞诱导为成熟的脂肪细胞后,运用ELISA技术检测培养液中IL-6,TNF-a,MCP-1等细胞炎症因子的变化。结果:一定浓度的FFA刺激脂肪细胞后,细胞培养上清液中FFA组IL-6,TNF-a,MCP-1三种因子浓度均会随时间变化升高(P0.05),其中FFA组TNF-a和MCP-1浓度在6小时、IL-6浓度在10小时升高(P0.05)。结论:FFA水平升高可致脂肪细胞多种炎症因子分泌加剧。
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| 关 键 词： | 肥胖 FFA 炎症因子 |
Effect of High Level of Free Fatty Acid on Adipocyte |
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| XU Wen-jing,ZHANG Jun,LU Huan,XIE Jing,LI Ai-Qin,ZHENG Li-Ying,LI Hong,XIE Jian-xin. Effect of High Level of Free Fatty Acid on Adipocyte[J]. Progress in Modern Biomedicine, 2010, 10(1) |
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| Authors: | XU Wen-jing ZHANG Jun LU Huan XIE Jing LI Ai-Qin ZHENG Li-Ying LI Hong XIE Jian-xin |
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| Affiliation: | XU Wen-jing,ZHANG Jun,LU Huan,XIE Jing,LI Ai-Qin,ZHENG Li-Ying,LI Hong,XIE Jian-xin(Department of Pathophysiology,Medical School/Laboratory of Xinjiang Endemic , Ethnic Diseases Shihezi University,Shihezi 832002 China) |
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| Abstract: | Objective:To investigate the effect of FFA on fat cells by collecting adipocyte culture medium at different time point after FFA stimulate adipocyte.Methods:ELISA technique was used to detect the IL-6,TNF-a,MCP-1 after 3T3-L1 preadipocyte differentiated into maturate adipocyte.Results:Inflammantry Cytokines IL-6,TNF-a,MCP-1 increased with time lasting(P<0.05) after stimulated by certain consentrate free fatty acid.TNF-a and MCP-1in group FFA consentrate increased at six hours while it is ten hours for IL-6.... |
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| Keywords: | obesity FFA Inflammantry Cytokines |
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