Deletion of the <Emphasis Type="Italic">CgTPI</Emphasis> Gene Encoding Triose Phosphate Isomerase of <Emphasis Type="Italic">Candida glycerinogenes</Emphasis> Inhibits the Biosynthesis of Glycerol |
| |
Authors: | Zhang Yongguang Shen Wei Rao Zhiming Fang Huiying Zhuge Jian |
| |
Institution: | (1) The Key Lab of Industrial Biotechnology of Ministry of Education, Research Centre of Industrial Microorganisms, School of Biotechnology, Jiangnan University, Wuxi, 214122, People’s Republic of China |
| |
Abstract: | The yeast Candida glycerinogenes produces a high yield of glycerol only in response to a medium-osmotic stress, but little is known about the relationship
between osmoadaptation and glycerol metabolism. The CgTPI gene encoding triose phosphate isomerase of C. glycerinogenes was cloned and sequenced, and its functionality was confirmed by complementation of Saccharomyces cerevisiae tpi1 Δ. The roles of CgTpip in the glycerol biosynthesis and the osmoadaptation were investigated. Unlike S. cerevisiae tpi1 Δ and Klyuveromyces lactis tpi1 Δ, the mutant lacking CgTPI significantly decreased the rate of glucose consumption and the glycerol yield. Furthermore, the mutants decreased osmotolerance
to glucose and NaCl. The results suggest that CgTPI might be crucial for a high yield of glycerol by C. glycerinogenes. The inhibition of glycerol biosynthesis might be related to the reduced ability of osmoadaptation to high external osmolarity.
To our knowledge, this is the first report that inactivation of a yeast TPI gene inhibits the biosynthesis of glycerol. |
| |
Keywords: | |
本文献已被 PubMed SpringerLink 等数据库收录! |
|