| 电子供体及受体对棕色固氮菌抗氨阻遏能力的影响 |
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| 引用本文： | 黄河清,孙耀东,林庆梅,唐华,翟文景. 电子供体及受体对棕色固氮菌抗氨阻遏能力的影响[J]. 微生物学通报, 2001, 28(4): 55-60 |
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| 作者姓名： | 黄河清 孙耀东 林庆梅 唐华 翟文景 |
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| 作者单位： | 环境科学研究中心 |
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| 基金项目： | 福建省自然科学基金资助项目(No.96006) |
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| 摘 要： | 电子供体连二亚硫酸钠，甲基紫精及电子受体亚甲蓝均能强烈的抑制棕色固氮菌表达固氮活性，并引起该菌的抗氨阻遏能力减弱，适当提高氧压，能提高菌体的固氮活性近15％，但过高的氧分压反而抑制菌体的固氮活性，此外，提高氢分压能降低棕色固氮菌菌体内的还原电位，从而达到提高菌体抗氨阻遏能力的效果。
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| 关 键 词： | 棕色固氮菌 抗氨阻遏 内环境电位 固氮活性 电子供体 电子受体 |
| 文章编号： | 0253-2654(2001)04-0055-06 |
| 修稿时间： | 2000-01-10 |
EFFECT OF ELECTRON DONOR AND ACCEPTOR ON CAPACITIES OF AMMONIA-RESITANT FROM AZOTBACTER VINELANDH |
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| HUANG He-Qing , SUN Yao-Dong LIN Qing-Mei TA NG Hua ZHAI Wen-Jing. EFFECT OF ELECTRON DONOR AND ACCEPTOR ON CAPACITIES OF AMMONIA-RESITANT FROM AZOTBACTER VINELANDH[J]. Microbiology China, 2001, 28(4): 55-60 |
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| Authors: | HUANG He-Qing SUN Yao-Dong LIN Qing-Mei TA NG Hua ZHAI Wen-Jing |
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| Affiliation: | HUANG He-Qing 1,3 SUN Yao-Dong 1 LIN Qing-Mei 2 TA NG Hua 1 ZHAI Wen-Jing 1 |
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| Abstract: | Electronic donors dithionite as well as m et hyl viologen and it receptor methylene blue strongly inhibited activity of nitro gen fixation that Azotobacter vinelandii expressed,which ca used capacity of ammonia-resistant weaken greatly.About 15% of total activity f rom the whole cell were risen by increasing suitable oxygen atmosphere.Opposite, t he activity was inhibited by increasing over concentration of O 2 further.The r eduction potential in the inside environment from Azotobacter vinelan dii whole cell shifted to negative by increasing hydrogen pressure,whi ch resulted in its capacity of ammonia-resistant rose. |
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| Keywords: | Azotobacter vinelandii Ammon ia-resistant Potential of inside environment Activity of nitrogen fixation |
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