Characterisation of a tetrasaccharide released on mild acid hydrolysis of LPS from two rough strains of Shewanella species representing different DNA homology groups |
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| Authors: | Moule Anne L Galbraith Lesley Cox Andrew D Wilkinson Stephen G |
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| Affiliation: | Department of Chemistry, University of Hull, Hull HU6 7RX, UK. |
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| Abstract: | A reducing tetrasaccharide of the following structure was released by mild acid hydrolysis of R-type LPS from Shewanella putrefaciens strains NCIMB 10472 and 10473. The same tetrasaccharide containing acetal-linked open-chain GalNAc is present in the core region of LPS from S. oneidensis strain MR-1 and may be characteristic of genomic groups II and III of S. putrefaciens and related strains. (1S)-d-GalaNAc-(1-->4,6)-alpha-d-Galp-(1-->6)-alpha-d-Galp-(1-->3)-d-Gal. |
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| Keywords: | LPS, lipopolysaccharide smallcaps" >dd-Hep, smallcaps" >d-glycero- smallcaps" >d-manno-heptose Kdo, 3-deoxy- smallcaps" >d-manno-oct-2-ulosonic acid 8-aminoKdo, 8-amino-3,8-dideoxy- smallcaps" >d-manno-oct-2-ulosonic acid GalaNAc, open-chain GalNAc. P, orthophosphate PP, pyrophosphate EtN, ethanolamine |
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