Molecular characterization of a specific p-nitrophenylphosphatase gene, PHO13, and its mapping by chromosome fragmentation in Saccharomyces cerevisiae |
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| Authors: | Yoshinobu Kaneko Akio Toh-e Isao Banno Yasuji Oshima |
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| Affiliation: | (1) Institute for Fermentation, Osaka, 2-17-85, Juso-honmachi, 532 Yodogawa-ku, Osaka, Japan;(2) Department of Biology, Faculty of Science, University of Tokyo, Hongo, 113 Bunkyo-ku, Tokyo, Japan;(3) Department of Fermentation Technology, Faculty of Engineering, Osaka University, 2-1 Yamadaoka, 565 Suita-shi, Osaka, Japan |
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| Abstract: | Summary The structural gene, PHO13, for the specific p-nitrophenyl phosphatase of Saccharomyces cerevisiae was cloned and its nucleotide sequence determined. The deduced PHO13 protein consists of 312 amino acids and its molecular weight is 34635. The disruption of the PHO13 gene produced no effect on cell growth, sporulation, or viability of ascospores. The PHO13 locus was mapped at 1.9 centimorgans from the HO locus on the left arm of chromosome IV. By chromosome fragmentation, the PHO13 locus was found to be located about 72 kb from the left-hand telomere of chromosome IV and distal to the HO locus. |
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| Keywords: | Chromosome fragmentation Mapping PHO13 sequence Phosphatase Yeast |
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