Methane formation from fructose by syntrophic associations of Acetobacterium woodii and different strains of methanogens |
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Authors: | Josef U. Winter Ralph S. Wolfe |
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Affiliation: | (1) Department of Microbiology, University of Illinois, 61801 Urbana, Illinois, USA;(2) Present address: Botanisches Institut der Universität München, Menzingerstr. 67, D-8000 München 19, Germany |
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Abstract: | When Acetobacterium woodii was co-cultured in continuous or in stationary culture with Methanobacterium strain AZ, fructose instead of being converted to 3 mol of acetate was converted to 2 mol of acetate and 1 mol each of carbon dioxide and methane, showing that interspecies hydrogen transfer occurred. In continous culture the organisms formed a close physical association in clumps; the doubling time for each organism was 6h at 33°C. Methane mainly was derived from carbon positions 3 and 4 of the sugar, but other carbons also yielded methane; this was shown to be due to carbon dioxide-acetate exchange reactions by A. woodii in a manner similar to that carried out by Clostridium thermoaceticum. Four other methanogens, Methanobacterium M.o.H. and M.o.H. G, Methanobacterium formicicum, and Methanosarcina barkeri (not acetate-adapted) also produced similar results, when co-cultured with A. woodii. |
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