Cloning and regulation of rat apolipoprotein B mRNA |
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| Authors: | A Matsumoto H Aburatani Y Shibasaki T Kodama F Takaku H Itakura |
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| Affiliation: | 1. National Institute of Nutrition Tokyo, Japan;7. The Third Department of Internal Medicine, University of Tokyo, Tokyo, Japan;1. Division of Vascular Surgery, Department of Surgery, University of Toronto, Toronto, Ontario, Canada;2. Institute of Health Policy, Management, and Evaluation, University of Toronto, Toronto, Ontario, Canada;3. Faculty of Medicine, University of Ottawa, Ottawa, Ontario, Canada;4. Temerty Faculty of Medicine, University of Toronto, Toronto, Ontario, Canada;5. Department of Surgery, University of Toronto, Toronto, Ontario, Canada;6. Peter Munk Cardiac Centre, University Health Network, Toronto, Ontario, Canada;7. Department of Vascular Surgery, McMaster University, Hamilton, Ontario, Canada;1. 1. Medizinische Klinik und Poliklinik, Klinikum Rechts der Isar, Technische Universität München, Munich, Germany;2. Institute for Cardiovascular Prevention, Ludwigs-Maximilians-Universität München, Munich, Germany;3. Deutsches Primatenzentrum GmbH, Leibnitz-Institut für Primatenforschung, Laboratory Animal Science Unit, Göttingen, Germany;4. DZHK (German Center for Cardiovascular Research), partner site Munich Heart Alliance, Munich, Germany;5. Institut für Pharmakologie und Toxikologie, Technische Universität München, Munich, Germany;6. Institute of Molecular and Translational Therapeutic Strategies, Hannover Medical School, Hannover, Germany;7. Department of Vascular Surgery, Klinikum Rechts der Isar, Technische Universität München, Munich, Germany;8. Department of Biochemistry, Cardiovascular Research Institute Maastricht, Maastricht University, Maastricht, the Netherlands;1. National Center for Cardiovascular Research (CNIC), Madrid, Spain;2. Department of Cardiology, Álvaro Cunqueiro University Hospital, Vigo, Spain;3. Centro de Investigación Biomédica en Red Consortium for Cardiovascular Diseases (CIBERCV), Madrid, Spain;4. Department of Cardiology, Son Espases University Hospital, Palma de Mallorca, Spain;5. Comprehensive Cardiovascular Diseases Center, HM Montepríncipe University Hospital, Madrid, Spain;6. Santander Bank, Madrid, Spain;7. Health Research Institute, October 12 Hospital (imas12), Madrid, Spain;8. Icahn School of Medicine at Mount Sinai, New York, New York, USA;9. Quirón University Hospital, Madrid, Spain;10. Health Research Institute Clínico San Carlos Hospital, Complutense University, Madrid, Spain;11. Health Research Institute, Fundación Jiménez Díaz University Hospital, Madrid, Spain;1. Universidad Regional Amazónica Ikiam, Parroquia Muyuna km 7 vía Alto Tena, 150150, Tena-Napo, Ecuador;2. Department of Organic and Inorganic Chemistry, University of Basque Country UPV/EHU, 48940, Leioa, Spain;3. Basque Center for Biophysics CSIC-UPVEH, University of Basque Country UPV/EHU, 48940, Leioa, Spain;4. IKERBASQUE, Basque Foundation for Science, 48011, Bilbao, Biscay, Spain;1. Fuwai Hospital, Chinese Academy of Medical Sciences, Beijing, China;2. Beijing Anzhen Hospital Affiliated to Capital Medical University, Beijing, China;3. Beijing Union Medical College Hospital, Beijing, China |
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| Abstract: | Recombinant cDNA clones that code for apolipoprotein B(apoB) were isolated from a rat liver cDNA library, using synthetic oligonucleotide probe derived from the sequence of human apoB cDNA. The nucleotide and deduced amino acid sequences of the rat apoB clone pRB5, 1.2 kb in length, showed 83% and 84% homology to those of human apoB. Northern blot analysis revealed that rat apoB cDNA probe cross-reacts with human and rabbit apoB mRNA sequences and the size of those mRNAs, approximately 15 kb long, were not discernibly different. In addition, apoB mRNA was abundant only in the liver and intestine. Finally, cholesterol feeding to rats for six weeks resulted in a several-fold increase in the level of apoB mRNA in the liver. |
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