Genetical analysis of in vitro cell and tissue culture response in potato |
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Authors: | M. Coleman R. Waugh W. Powell |
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Affiliation: | (1) Cellular and Molecular Genetics Department, Scottish Crop Research Institute, Invergowrie, DD2 5DA Dundee, Scotland |
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Abstract: | The genetics of tissue culture response in potato has been examined by analysing a sample of dihaploids (2n=2x=24) extracted from tetraploid parents (4n=4x=48). The genotypes were screened for rate of nodal multiplication, in vitro tuberisation, regeneration from leaf discs and protoplast plating efficiency. Significant differences were detected between dihaploids for the traits measured and this indicates that tissue culture response in the tetraploid parents must be in the heterozygous condition. Estimates of the broad sense heritabilities were calculated together with the number of genes or effective factors involved in the control of the traits. These estimates indicate that tissue culture response in potato is under relatively simple genetic control and blocks of genes may be located on specific chromosomes. The inheritance of RFLP markers in the segregating dihaploid population was also monitored and the potential of using molecular markers linked to gene(s) controlling tissue culture response is discussed. |
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Keywords: | heritability protoplasts RFLPs Solanum tuberosum |
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